In enzyme washing, cellulase enzymes are used. Hydrolysis of the
cellulose, which is catalysed by cellulase, causes the surface fibres to become
weakened and later they
get removed when there is either fabric-to-fabric
abrasion or fabric-to-stone abrasion during washing. The temperature and the pH used
are specific to the type of cellulase employed. Usually neutral cellulases are
applied at pH 6–7, while acid cellulases are applied at pH 4.5–5.5.
However, the latter result in a greater extent of back staining,
being more penetrative. An enzyme dose of 2–4 g/L is normally enough, provided
that the enzyme activity is not impaired. In general, the colour of the enzyme
washed goods is more uniform, particularly when stone is not added. Because cellulases
are reactive only on cellulose, any size materials or other impurities must be removed
before the cellulase treatment.